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The curd and buttermilk are enriched with a lot amount of lactic acid bacteria. Honey has previously been shown to have wound healing and antimicrobial properties, but this is dependent on the type of honey. Antimicrobial agents are essentially important in reducing the global burden of infectious diseases.

Panchagavya is a term used in Ayurveda to describe five important substances obtained from cow namely Urine, Dung, Milk, casey deidrick dating Ghee Curd and the natural product of honey. Indian cow urine is very powerful medicine in the Ayurvedic side. The present study analyzes the antibacterial activity of the natural product.

Muller Hinton agar medium plates were prepared and sterilized. High osmolality, low pH, and hydrogen peroxide are the main antimicrobial factors.

The ancient Egyptians made offerings of honey to their gods used it as an embalming fluid and dressing for wounds. The use of honey as a traditional remedy for microbial infections dates back to ancient times. Also, phenolic compounds may contribute to the antimicrobial activity. Cow urine from different cows had a different level of antimicrobial properties.

All the isolates were tested for their antibiotic susceptibility. Antibiotic sensitivity test S. Several properties in honey contribute to its antimicrobial activity.

There is a considerable zone of inhibition for cow dung was observed only in Staphylococcus aureus. The urine sample was brought to the laboratory in a sterile container within one hour after the collection and processed immediately.

Both broad spectrum and narrow spectrum antibiotics were used. The sample used as a different concentration of agar well diffusion technique. Different products obtained from cow milk, ghee, curd, urine, and dung are used widely in a number of Ayurvedic formulations. Processes of cow urine sample Fresh cow urine was collected in sterile screw cap bottles and brought to the laboratory for testing. Honey has a long medicinal history.

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The honey, when applied topically, rapidly clears wound infection to facilitate healing of deep surgical wounds with infection. The crude extract of Lactobacillussp. The predominant among the isolates were Escherichia coli and Staphylococcus aureus. There is absence zone of inhibition was observed in cow urine and ghee.

Among the natural products honey, milk, buttermilk was effective. Today, many people swarm to honey for its antibacterial and anti-inflammatory properties. Antibacterial activity of cow urine, milk, ghee, buttermilk curd and honey for the bacterial isolates has been determined by agar well diffusion method and the sensitivity pattern. It was filtered by ordinary filter paper before being subjected to further testing.

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